Fluorometry is a method of identifying and analyzing samples based on the light absorbed and then emitted from a sample; fluorescence.
In fluorescence spectroscopy, the amount of light emitted after absorption is measured to give information on the components of the sample.
Applications of Fluorescence Spectroscopy:
Range of Fluorescence Spectroscopy Instrumentation:
There are several types of spectrometers used in fluorometry. Filter fluorometers, which pass light through filters, and spectrofluorometers, which pass light through diffraction grating monochromators. See below other types of systems, including fluorescence lifetime spectrometers and fluorescent readers.
What to consider when choosing a fluorescence spectrometer?
Before purchasing a fluorometer, keep in mind the parameters that are best for your needs, including wavelengths that it can filter and analyze, time to run analysis, and sample size needed.
Latest advances of Fluorescence Spectroscopy:
There are some exciting new uses of fluorescence spectroscopy being developed. For example, fluorescent nanosensors for oxygen have been developed that allow the measurement of oxygen in biological fluids such as blood, interstitial fluid, and cerebral spinal fluid.
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